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We find that this works best with two people. One person squeezes eggs from
females while the other person thaws the sperm.
1. Set a water bath to 33ºC.
2. Remove cryovial from liquid nitrogen freezer and transfer to liquid
nitrogen-containing dewar flask until ready to thaw.
3. Squeeze eggs from females into 35mm plastic petri dish. If possible, try to
obtain 3 clutches of eggs and combine into one dish. If you can not get
three good clutches within 1 min of your first clutch, then proceed to step 4
(one good clutch is sufficient). Definition of good clutch: >150 uniformly
nice looking eggs (i.e. yellowish, with no white debris indicative of
degradation). Keep dish covered while sperm is thawed.