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Principle of the assay: This assay will test specific interactions between proteins and
immobilized lipids. It is essentially most similar to immunoblotting, with the added step of protein
binding preceding the antibody steps.
Special reagents and equipment:
Fatty Acid-Free (FAF) BSA Sigma Cat# A6003
Hybond C extra membrane Amersham Biosciences Cat # RPN2020E