1) 0.1 M phosphate buffer, pH 7.2-7.4 (store in cold room)
2) 12% paraformaldehyde (store at -20)
10.8 g paraformaldehyde in 90 ml ddH2O, heat/stir and add NaOH to solve
3) 0.2 M Lysine HCl
7.32 g Lysine HCl in 200 ml of 0.1 M PB
4) PLP (4% paraformaldehyde)
1X 2X 4X 4.5X 7X 8X
Phosphate buffer 50 100 200 225 350 400 ml
0.2 M Lysine HCl 50 100 200 225 350 400 ml
12% paraformaldehyde 33.3 66.6 133.2 149.8 233.1 266.4 ml
Sodium Metaperiodate 0.3 0.6 1.2 1.35 2.1 2.4 g
Adjust pH to 7.4 with 1M NaOH or HCl