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Note: Use a negative control with non-BrdU treated cells.
Required Solutions
BrdU/BrdC
BrdU (MP Biomedicals 100166) stock concentration: 10 mM in H2O
BrdC (Sigma B5002) stock concentration: 10 mM in H2O
Make 1000x working stock solution of BrdU/BrdC by mixing 3 parts BrdU to 1 part BrdC (final
concentrations of 7.5 mM of BrdU and 2.5 mM of BrdC.)
Store at -20°C covered in aluminum foil.
Colcemid (Roche 10295892001)
Stock solution is 1 mg/ml. Dilute in DMEM to required concentration. Store at -20°C.
Use 0.2 ug/ml for mouse cells and 0.1 ug/ml for human cells.